Suitability of reverse micelles of anionic surfactant sodium bis(2-ethyl hexyl) sulfosuccinate (AOT) and sodium dodecyl sulfate (SDS), cationic surfactant hexadecyl trimethyl ammonium bromide (CTAB) and nonionic surfactant polyoxyethylene p-t-octylphenol (TritonX-100) in organic solvent isooctane for extraction of soy isoflavone-enriching proteins was investigated. The results showed that the order of combined isoflavone contents was SDS > CTAB > Triton X-100 > AOT, while the order of protein recovery was SDS > AOT > TritonX-100 > CTAB. As compared with ACN-HCl extraction, the total amount of isoflavones was lower than reverse micellar extraction. Ion strength was one of the important conditions to control extraction of isoflavone-enriching proteins with AOT reversed micelles. For the six salt systems, KNO3, KCl, MgCl2, CaCl2, NaCl, and Na2SO4, extracted fraction of isoflavone-enriching proteins was measured. Salt solutions greatly influenced the extraction efficiency of isoflavones in an order of KNO3 > MgCl2 > CaCl2 > KCl > NaCl > Na2SO4, while protein in an order of MgCl2 > CaCl2 > NaCl > KNO3 > Na2SO4 > KCl.