The process of galanthamine and related alkaloids production by Leucojum aestivum shoot culture in a temporary immersion system was studied. It was established that temporary immersion approach is prospective for development of a biosynthetic process for obtaining valuable Amaryllidaceae alkaloids. Both immersion frequency and temperature had significant effect on biomass accumulation and the yields of galanthamine and related alkaloids. The maximal yield of galanthamine was achieved at the cultivation of L. aestivum shoot culture in temporary immersion RITA(A (R)) system at immersion frequency 15 min flooding and 8 h stand-by periods, at 26 A degrees C. Data on the relationships in the biological system "Nutrient medium-L. aestivum shoot culture-galanthamine" are presented as well.