Coordinated Nodal-related signals and Bozozok (Boz) activity are critical for the initial specification of dorsal mesoderm and anterior neuroectoderm during zebrafish embryogenesis Overexpression of Boz expands gsc expression into the ventro-lateral marginal blastomeres where Nodal signaling is active but is insufficient to Induce ectopic gsc expression in the animal region We found that overexpression of Boz together with depletion of Lnx-2b (previously named Lnx-like Lnx-1) but not each manipulation alone causes robust gsc expression in all blastomeres Furthermore nodal-related signals are required for gsc expression in embryos with elevated Boz activity Through targeted injection into single cells at the 128-cell stage we illustrate the role of maternally deposited Lnx-2b to restrict the expansion of gsc expression into the presumptive ectodermal region This report provides a novel mechanism for limiting dorsal organizer specification to a defined region of the early zebrafish embryo Published by Elsevier Inc