The glycosynthase derived from Bacillus licheniformis 1,3 1,4-beta-glucanase was able to polymerize glycosyl fluoride donors (G4)(m)G3G alpha F (m = 0-2, G = Glc beta) leading to artificial mixed-linked beta-glucans with regular sequences and variable beta 1,3 to beta 1,4 linkage ratios. With the E134A glycosynthase mutant, polymers had average molecular masses (M-w) of 10-15 kDa. Whereas polymer 2 ([4G4G3G](n)) was an amorphous precipitate, the water-insoluble polymers 1 ([4G3G](n)) and 3 ([4G4G4G3G](n)) formed spherulites of 10-20 mu m diameter. With the more active E134S glycosynthase mutant, polymerization led to high molecular mass polysaccharides, where M-w was linearly dependent on enzyme concentration. Remarkably, a homo-polysaccharide [4G4G4G3G](n) with M-w as high as 30.5 kDa (n approximate to 47) was obtained, which contained a small fraction of products up to 70 kDa, a value that is in the range of the molecular masses of low viscosity cereal 1,3-1,4-beta-glucans, and among the largest products produced by a glycosynthase. Access to a range of novel tailor-made beta-glucans through the glycosynthase technology will allow to evaluate the implications of polysaccharide fine structures in their physicochemical properties and their applications as biomaterials, as well as to provide valuable tools for biochemical characterization of beta-glucan degrading enzymes and binding modules.