Ensiling of humid biomass samples wrapped in plastic bales has been investigated as a wet-storage for bioethanol production from three lignocellulosic biomass samples i.e. maize, rye, and clover grass. During the silage process, lactic acid bacteria fermented free sugars to lactic acid, and consequently by lowing pH, inhibiting other microbes to degrade the polysaccharides. Following silage treatment, enzymatic convertibility tests showed that 51.5%, 36.5%, and 41.9% of the cellulose was converted by cellulytic enzymes in ensiled maize, rye, and clover grass, respectively. In addition, tests of SSF (simultaneous saccharification and fermentation) were carried out using combined enzymatic hydrolysis and fermentation with two different microorganisms Saccharomyces cerevisiae and Kluyveromyces marxianus. The ethanol production was 33.0%, 27.5%, 36.9% (by K. marxianus) and 29.0%, 28.1%, 34.5% (by S. cerevisiae); the yields significantly increased after hydrothermal pretreatment: 77.7%, 72.8%, 79.5% (by K. marxianus) and 72.0%, 80.7%, 75.7% (by S. cerevisiae) of the theoretical based on the C6 sugar contents in maize, rye, and clover grass, respectively. (C) 2011 Elsevier Ltd. All rights reserved.