화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.19, No.12, 1250-1256, December, 2011
DOI10.1007/s13233-011-1203-3  Export Citation
Morphological Property and In vitro Enzymatic Degradation of Modified Chitosan as a Scaffold
In-Ho Bae1, 2, Won Gu Jang1, 3, Hyun-Pil Lim1, 3, Sang-Won Park1, 3, Kwang-Min Lee4, Young-Joon Park1, 3, In-Kyu Park5, 2, Myung Ho Jeong2, and Jeong-Tae Koh1, 3, †
  • 1Dental Science Research Institute and BK21 Project, School of Dentistry, Chonnam National University, Gwangju, 500-757, Korea
  • 2Heart Research Center, Chonnam National University Hospital, Gwangju, 501-757, Korea
  • 3, Korea
  • 4Division of Materials Science and Engineering, Chonnam National University, Gwangju, 500-757, Korea
  • 5Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju, 501-746, Korea
E-mail:
Chitosan (CS) was proposed as a promising candidate scaffold for tissue engineering. However, some drawbacks of natural CS remain. The current study modified CS by conjugating thiol to CS polymer (Thio-CS) and substantiated its three-dimensional microstructure and physical properties such as swelling or degradation. The Thio-CS was obtained by CS modification using 2-iminothiolane-HCl (2-IT). Because of the formation of disulfide bonds between thiol moieties based on oxidation of the immobilized thiol groups of CS, Thio-CS exhibits in situ gelling properties according to the reducing amount of free thiol. The content of the thiol group was increased as the amount of 2-IT increased. The swelling test demonstrated that Thio-CS can absorb up to 3.5 times its weight of phosphate buffered saline within 1 h and that the pore size and amount significantly increased with incubation time. The Thio-CS enzymatic degradation rate according to velocity was investigated. The results showed that Thio-CS was more resistant to lysozyme as viscosity increased. Thio-CS sponges were fabricated using freezedrying. The lyophilized Thio-CS had a homogeneous honeycomb-like shape, and its pores were relatively smaller (<2 μm) than those of unmodified CS (>2 μm). These results suggest that Thio-CS might be a candidate regenerative therapeutic device.
Keywords:modified chitosan;scaffold;thiolation;morphological property;enzymatic degradation.
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