화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.29, No.2, 221-227, February, 2012
DOI10.1007/s11814-011-0165-7  Export Citation
Physicochemical properties and bioactivity of brown seaweed fucoidan prepared by ultra high pressure-assisted enzyme treatment
Keunhyoung Park, Eunhye Cho, Man-Jin In1, Dong Chung Kim1, and Hee Jeong Chae†
  • Department of Food and Biotechnology and Natural Choice Co., Ltd., Hoseo University, Asan 336-795, Korea
  • 1Department of Human Nutrition and Food Science, Chungwoon University, Hongseong 350-701, Korea
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The effects of ultra-high pressure (UHP, 100 MPa, 40, 24 h) processing and enzyme treatment on the physicochemical properties and anticoagulant activity of fucoidan obtained from Undaria pinnatifida sporophyll (UPS) were investigated. Crude UPS fucoidan (FUPS) with an average MW of 877 kDa was slightly depolymerized by UHPassisted Tunicase treatment, yielding fucoidan with a lower MW (600-800 kDa). UHP-enzyme treatment decreased the sulfate, fucose and galactose contents of FUPS but increased its glucose content. From FT-IR spectrum, UHP-enzyme treatment was found not to cause a structural change on S=O and C-O-S. After UHP-enzyme treatment, the sulfate content and average MW of FUPS decreased with the increase of Tunicase concentration. It was found that among the UHP-enzyme-treated fucoidans, FUPS-T0.3-U (Tunicase 0.3% treatment+UHP treatment) possessed the highest anticoagulant activity. FUPS-T0.3-U appeared to inhibit blood coagulation via intrinsic pathway. With the increase of sulfate content in FUPS, activated partial thromboplastin time (APTT) and thrombin time (TT) showed a tendency of increase. APTT and TT had the highest values in FUPS-T0.3-U in which the sulfate concentration was 24%, but on the contrary decreased at a sulfate concentration of above 24%. This result indicates that there is an optimum sulfate concentration for the anticoagulant activity of fucoidan. Consequently, UHP-assisted enzymatic treatment was found to be helpful for the improvement of anticoagulant activities of fucoidan.
Keywords:Fucoidan;Ultra High Pressure Processing;Enzyme Treatment;Sulfate Content;Bioactivity;APTT;TT
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