Characterization of oil including astaxanthin extracted from krill (Euphausia superba) using supercritical carbon dioxide and organic solvent as comparative method

Abdelkader Ali-Nehari; Seon-Bong Kim; Yang-Bong Lee; Hye-youn Lee; Byung-Soo Chun

Korean Journal of Chemical Engineering, Vol.29, No.3, 329-336, March, 2012

DOI10.1007/s11814-011-0186-2 Export Citation

Characterization of oil including astaxanthin extracted from krill (Euphausia superba) using supercritical carbon dioxide and organic solvent as comparative method

Abdelkader Ali-Nehari, Seon-Bong Kim¹, Yang-Bong Lee¹, Hye-youn Lee¹, and Byung-Soo Chun^{1, †}

International Program of Fisheries Sciences, Faculty of Fisheries Sciences, Pukyong National University, 599-1, Daeyeon-3dong, Nam-gu, Busan 608-737, Korea
¹Department of Food Science and Technology, Faculty of Fisheries Sciences, Pukyong National University, 599-1, Daeyeon-3dong, Nam-gu, Busan 608-737, Korea

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Krill oil including astaxanthin was extracted using supercritical CO2 and hexane. The effects of different parameters such as pressure (15 to 25MPa), temperature (35 to 45 ℃), and extraction time, were investigated. The flow rate of CO2 (22 gmin.1) was constant for the entire extraction period of 2.5 h. The maximum oil yield was found at higher extraction temperature and pressure. The oil obtained by SC-CO2 extraction contained a high percentage of polyunsaturated fatty acids, especially EPA and DHA. The acidity and peroxide value of krill oil obtained by SC-CO2 extraction were lower than that of the oil obtained by hexane. The SC-CO2 extracted oil showed more stability than the oil obtained by hexane extraction. The amount of astaxanthin in krill oil was determined by HPLC and compared at different extraction conditions. The maximum yield of astaxanthin was found in krill oil extracted at 25 MPa and 45 ℃.

Keywords:Krill;Oil;Astaxanthin;Supercritical Carbon Dioxide Extraction;Hexane

[References]

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Sun M, Temelli F, J. Supercrit. Fluids, 37(3), 397 (2006)
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AOCS, American Oil Chemists’ Society, Washington DC, USA (1992)
Illes V, Daood HG, Biacs PA, Gnayfeed MH, Meszaros B, J. Chromatogr. Sci., 37, 345 (1999)
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Park JY, Lee MK, Uddin MS, Chun BS, Biotechnol. Bioprocess Eng., 13, 298 (2008)
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[Referenced By]

[1] Yamaguchi K, Murakami M, Nakano H, Konosu S, Kokura T, Yamamoto H, Kosaka M, Hata K, J. Agric. Food Chem., 34, 904 (1986)

[2] Shahar E, Folsom AR, Melnick SL, Comstock GW, Gennaro V, Higgins MW, Sorlie PD, Ko WJ, Szklo M, N.Eng. J. Med., 331, 228 (1994)

[3] Correa APA, Peixoto CA, Goncalves LAG, Cabral FA, J. Food Eng., 88, 381 (2008)

[4] Li HB, Chen F, J. Chromatogr. A., 925, 133 (2001)

[5] Lopez M, Arce L, Garrido J, Rios A, Valcarcel M, Talanta., 64, 726 (2004)

[6] Sun M, Temelli F, J. Supercrit. Fluids, 37(3), 397 (2006)

[7] Shimidzu N, Goto M, Miki W, Fish Sci., 62, 134 (1996)

[8] AOCS, American Oil Chemists’ Society, Washington DC, USA (1992)

[9] Illes V, Daood HG, Biacs PA, Gnayfeed MH, Meszaros B, J. Chromatogr. Sci., 37, 345 (1999)

[10] Careri M, Furlattini L, Mangia A, Musci M, Anklam E, Theobald A, Holst CV, J. Chromatogr. A., 912, 61 (2001)

[11] Mendes RL, Nobre BP, Cardoso MT, Pereire AP, Palavre AF, Inorg. Chim. Acta., 356, 328 (2003)

[12] Lim GB, Lee SY, Lee EK, Haam JS, Kim WS, Biochem.Eng. J., 11, 181 (2002)

[13] Sahena F, Zaidul ISM, Jinap S, Yazid AM, Khatib A, Norulaini NAN, Food Chem., 120, 879 (2010)

[14] Rizvi SSH, Chao RR, Liaw YJ, In supercritical fluid extraction and chromatography: Techniques and applications, ACS Washington, USA (1988)

[15] Temelli F, Leblanc E, Fu L, J. Food Sci., 60, 703 (1995)

[16] Pawliszyn J, J. Chromatogr. Sci., 31, 31 (1993)

[17] AOCS, American Oil Chemists’ Society, Champaign, Illinois, USA

[18] AOCS, American Oil Chemist’s Society, Physical and Chemical Characteristics of Oils, Fats and Waxes Table of Contents, 2, 54 (1997)

[19] Bernardez M, Pastoriza L, Sampedro G, Herrera JJR, Cabo ML, J. Agri. F. Chem., 53, 1903 (2005)

[20] Lowry RR, Tinsley IJ, J. Am. Oil Chem. Soc., 7, 470 (1976)

[21] Krichnavaruk S, Shotipruk A, Goto M, Pavasant P, Bioresour.Technol., 99, 5556 (2008)

[22] Ooi CK, Bhaskar A, Yener MS, Tuan DQ, Hsu J, Rizvi SSH, J. AOCS., 73, 223 (1996)

[23] Morita A, Kajimoto O, J. Phys. Chem., 94, 6420 (1990)

[24] de Azevedo ABA, Kieckbush TG, Tashima AK, Mohamed RS, Mazzafera P, de Melo SABV, J. Supercrit. Fluids, 44(2), 186 (2008)

[25] Park JY, Lee MK, Uddin MS, Chun BS, Biotechnol. Bioprocess Eng., 13, 298 (2008)

[26] Sanchez-Vicente Y, Cabanas A, Renuncio JAR, Pando C, J. Supercrit. Fluids, 49(2), 167 (2009)

[27] Rubio-Rodriguez N, de Diego SM, Beltran S, Jaime I, Sanz MT, Rovira J, J. Supercrit. Fluids, 47(2), 215 (2008)

[28] Kamal-Eldin A, Yanishlieva NV, Eu. J. Lip. S. Technol., 104, 825 (2002)

[29] Miki W, Toriu N, Kondo Y, Murakani M, Yamaguchi K, Konosu S, Satake M, Fujita T, Nippon Suisan Gakkaishi., 49, 1417 (1983)

[30] Thana P, Machmudah S, Goto M, Sasaki M, Pavasant P, Shotipruk A, Bioresour. Technol., 99(8), 3110 (2008)

[31] Chrastil J, J. Phys. Chem., 86, 3016 (1982)

[32] Wahyu BS, Nawi AHM, Omar AK, Int. C. En. R. Tech., 921 (2008)

[33] Roh MK, Uddin MS, Chun BS, Biotechnol. Bioprocess Eng., 13, 724 (2008)