Polyglycidylmethacrylate beads with macroporous structure were synthesized by suspension polymerization and directly used for Candida species 99-125 lipase immobilization because of their rich surface epoxy groups. The properties of the synthesized polymer beads were characterized by scanning electron microscopy and FT-IR spectroscopy. To increase the enzyme activity, divinyl benzene and toluene/heptane were used as cross-linker and porogen, respectively, and the effects of cross-linker content and porogen content on lipase immobilization were studied. After optimizing the synthesis conditions, the optimum lipase concentration for immobilization was determined as 10 mg mL(-1). The immobilized lipase showed a broader pH stability and higher temperature stability. The immobilized enzyme could be reused for ten batches with 62% of residual activity.