Carbon nanotubes (CNTs) were produced by gas phase two-stage chemical vapor deposition using ferrocene as a catalyst and acetylene (C2H2) and hydrogen (H-2) as precursor gases. The CNTs were characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), and by thermogravimetric analysis (TGA). Process parameters such as reaction temperature, reaction time and gas flow rates of C2H2 and H-2 were optimized using Design-Expert software v.6.0.8. The statistical analysis reveals that the optimum conditions for the CNT production were reaction temperature of 850 degrees C, reaction time of 60 min and gases flow rates of 40 and 150 ml/min for C2H2 and H-2. respectively. The optimum CNTs prepared were purified and functionalized using acid treatment. Both functionalized and nonfunctionalized CNTs were used as column chromatographic media for skim latex protein purification. Skim latex protein was used as the model protein for the recovery of useful proteins and enzymes. During purification, process parameters such as pH and ionic strength of the running buffer were optimized to enhance protein purification. Results revealed that CNTs behave efficiently as a hydrophobic interaction chromatographic (HIC) media. (C) 2011 Elsevier B.V. All rights reserved.