Chemical Physics Letters, Vol.503, No.4-6, 310-315, 2011
Low-power homonuclear dipolar recoupling using supercycled symmetry-based and exponentially-modulated pulse sequences
Aimed at performing dipolar recoupling as part of biological solid-state NMR experiments without using excessively strong rf irradiation, we present a series of low-power supercycled symmetry-based and exponentially modulated pulse sequences facilitating efficient recoupling over specified chemical shift ranges even at high-speed spinning conditions. The experiments are designed through simple phase modulation of known 'lower-quality' symmetry-based pulse sequences or highly rf-demanding exponentially modulated recoupling experiments. The methods are described analytically and numerically and demonstrated experimentally by one-and two-dimensional correlation spectra of 2,3-C-13(2)-L-alanine and the fibrillating-core decapeptide SNNFGAILSS of human Islet Amyloid Poly-Peptide (hIAPP, or amylin) uniformly C-13, N-15-labeled at the FGAIL residues. (C) 2010 Elsevier B. V. All rights reserved.