In this study, we investigated the possibility of employing an insulin amyloid without the cell binding motif sequence as a novel cell adhesion material. Immobilized insulin amyloid on cell culture plates shows higher cell adhesion properties compared with untreated plates. Interestingly, cells adhered to the insulin amyloid displayed marked cell proliferation. It was also demonstrated that the insulin amyloid interacted with fibronectin present within the serum.