Effective recovery of UO22+ from wastewater is essential for nuclear fuel industry and related industries. In this study, a novel adsorbent was prepared by loading titanium (Ti4+) onto collagen fiber (TICF), and its physical and chemical properties as well as adsorption to UO22+ in nuclear fuel industrial wastewater were investigated. It is found that TICF can effectively recover UO22+ from the wastewater with excellent adsorption capacity. The adsorption capacity is 0.62 mmol.g(-1) at 303 K and pH 5.0 when the initial concentration of UO22+ is 1.50 mmol.L-1. The adsorption isotherms can be described by the Langmuir equation and the adsorption capacity increases with temperature. The effect of co-existed F- on the adsorption capacity for UO22+ is significant, which can be eliminated by adding aluminum ions as complexing agent, while the other co-existed ions in the solutions, including HCO3-, Cl-, NO3-, Ca2+, Mg2+ and Cu2+, have little effect on the adsorption capacity for UO22+. The saturated TICF after UO22+ adsorption can be regenerated by using 0.2 mol.L-1 nitrate (HNO3) as desorption agent, and the TICF can be reused at least three times. Thus the TICF is a new and effective adsorbent for the recovery of UO22+ from the wastewater.