Ethylcellulose microparticles containing chitosan and gelatin: pH-dependent release caused by complex coacervation

Mi Kyoung Kang; Jing Dai; Jin-Chul Kim

Journal of Industrial and Engineering Chemistry, Vol.18, No.1, 355-359, January, 2012

DOI10.1016/j.jiec.2011.11.099 Export Citation

Ethylcellulose microparticles containing chitosan and gelatin: pH-dependent release caused by complex coacervation

Mi Kyoung Kang, Jing Dai, and Jin-Chul Kim^†

Division of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2-dong, Chunchon, Kangwon-do 200-701, Republic of Korea

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Ethylcellulose microparticles containing chitosan and gelatin were prepared by spray drying water-in-oil (W/O) emulsion. Water phase was chitosan:gelatin solution in distilled water, and oil phase was ethylcellulose solution in dichloromethane. FITC-dextran was included in water phase as a fluorescence dye. The maximum coacervation was observed at pH 6.0 when the ratio of chitosan to gelatin was 1:15. Microparticles containing chitosan and gelatin in the ratio showed a higher release under acidic condition (e.g. pH 4.0) and neutral condition (e.g. pH 7.0), but a lower release at pH 5.0 and pH 6.0. The complex coacervate composed of chitosan and gelatin was efficiently formed at those pHs, and the formation of coacervate would be responsible for the lower release.

Keywords:Microparticles;Chitosan;Gelatin;Complex coacervation;Release

[References]

Wyss A, Cordente N, von Stockar U, Marison IW, Biotechnol. Bioeng., 87(6), 734 (2004)
Greene LC, Meyers PA, Springer JT, Banks PA, J. Agric. Food Chem., 40, 2274 (1992)
Taylor NJ, Fauquet CM, DNA Cell Biol., 21, 963 (2002)
Park DJ, Jackson WR, McKinnon RI, Marshall M, in: Scher HB (Ed.), Controlled-Release Delivery Systems for Pesticides, CRC Press, New York, 89 (1999)
Soda ME, Pannell L, Olson N, J. Microencapsul., 6, 319 (1989)
Patravale VB, Mandawgade SD, J. Cosmet. Sci., 30, 19 (2008)
Suares AJ, Dobkowski BJ, 1995, USP 5,612,044.
Soest JJG, in: Berger RG (Ed.), Flavours and Fragrances . Chemistry, Bioprocessing and Sustainability, Springer, New York, 439 (2007)
Yu CY, Yin BC, Zhang W, Cheng SX, Zhang XZ, Zhuo RX, Colloid Surf. B Biointerfaces., 68, 245 (2009)
Lynn DM, Amiji MM, Langer R, Angew. Chem. Int. Ed., 40, 1707 (2001)
Kim BY, Kang HS, Kim JD, J. Microencapsul., 19, 661 (2001)
Yin R, Han J, Zhang J, Nie J, Colloid Surf. B Biointerfaces., 76, 483 (2010)
Cheng J, Teply BA, Jeong SY, Yim CH, Ho D, Sherifi I, Jon S, Farokhzad OC, Khademhosseini, R.S. Langer RS, Pharm. Res., 23, 557 (2006)
Inmaculada A, Mengibar M, Ruth H, Panos I, Beatriz M, Niuris A, Gemma G, Angeles H, Curr. Chem. Biol., 3, 203 (2009)
Rinaudo M, Prog. Polym. Sci., 31, 603 (2006)
Alleavitch J, Turner WA, Finch CA, in: Elvers B, Hawkins S, Ravenscroft M, Rounsaville JF, Schulz G (Eds.), Ullmann’s Encyclopedia of Industrial Chemistry, vol. A12. VCH, New York, 307 (2010)
Kang MK, Kim JC, J. Appl. Polym. Sci., 118(1), 421 (2010)

[Referenced By]

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[1] Wyss A, Cordente N, von Stockar U, Marison IW, Biotechnol. Bioeng., 87(6), 734 (2004)

[2] Greene LC, Meyers PA, Springer JT, Banks PA, J. Agric. Food Chem., 40, 2274 (1992)

[3] Taylor NJ, Fauquet CM, DNA Cell Biol., 21, 963 (2002)

[4] Park DJ, Jackson WR, McKinnon RI, Marshall M, in: Scher HB (Ed.), Controlled-Release Delivery Systems for Pesticides, CRC Press, New York, 89 (1999)

[5] Soda ME, Pannell L, Olson N, J. Microencapsul., 6, 319 (1989)

[6] Patravale VB, Mandawgade SD, J. Cosmet. Sci., 30, 19 (2008)

[7] Suares AJ, Dobkowski BJ, 1995, USP 5,612,044.

[8] Soest JJG, in: Berger RG (Ed.), Flavours and Fragrances . Chemistry, Bioprocessing and Sustainability, Springer, New York, 439 (2007)

[9] Yu CY, Yin BC, Zhang W, Cheng SX, Zhang XZ, Zhuo RX, Colloid Surf. B Biointerfaces., 68, 245 (2009)

[10] Lynn DM, Amiji MM, Langer R, Angew. Chem. Int. Ed., 40, 1707 (2001)

[11] Kim BY, Kang HS, Kim JD, J. Microencapsul., 19, 661 (2001)

[12] Yin R, Han J, Zhang J, Nie J, Colloid Surf. B Biointerfaces., 76, 483 (2010)

[13] Cheng J, Teply BA, Jeong SY, Yim CH, Ho D, Sherifi I, Jon S, Farokhzad OC, Khademhosseini, R.S. Langer RS, Pharm. Res., 23, 557 (2006)

[14] Inmaculada A, Mengibar M, Ruth H, Panos I, Beatriz M, Niuris A, Gemma G, Angeles H, Curr. Chem. Biol., 3, 203 (2009)

[15] Rinaudo M, Prog. Polym. Sci., 31, 603 (2006)

[16] Alleavitch J, Turner WA, Finch CA, in: Elvers B, Hawkins S, Ravenscroft M, Rounsaville JF, Schulz G (Eds.), Ullmann’s Encyclopedia of Industrial Chemistry, vol. A12. VCH, New York, 307 (2010)

[17] Kang MK, Kim JC, J. Appl. Polym. Sci., 118(1), 421 (2010)