Laminar burning velocities and Markstein lengths of 2,5-dimethylfuran (DMF)-air premixed mixtures at different initial temperatures and equivalence ratios were obtained using a constant volume combustion chamber and high-speed schlieren imaging system. The results indicate that both unstretched flame propagation speed and laminar burning velocity peak near the equivalence ratio of 1.2, and increase with increasing initial temperature. The peak unstretched flame propagation speed moves to the rich mixture side as the initial temperature increases, whereas the peak laminar burning velocity is unaffected by the variation of initial temperature. The Markstein length decreases with increasing equivalence ratio and increases with the increasing initial temperature. Based on the experimental data, a formula to calculate the laminar burning velocities of 2,5-DMF-air mixtures is provided.