In this study, amino group modified mesoporous silica nanoparticles (MSN) were prepared and used to immobilize both platinum nanoparticles (PtNP) and glucose oxidase (GOx). The prepared MSN-PtNP demonstrated high stability and reactivity for catalyzing H2O2 electro-reduction, mainly due to the large amount of PtNP immobilized, the high surface area of these catalysts and the unique nanostructures formed through the synthetic route. Working at -0.2 V. the linear range in response to H2O2 by the prepared MSN-PtNP can be 5 x 10(-7) to 6 x 10(-2) MOI L-1 After immobilizing GOx onto MSN-PtNP, the resulting MSN-PtNP-GOx was capable of interference-free determination of glucose with the linear range as wide as 1 x 10(-6) to 2.6 x 10(-2) mol L-1. Furthermore, the fabricated glucose biosensor can offer significant advantages compared with its conventional counterparts, typically like the high sensitivity, good reproducibility and stability, and rapid response ability as well. The fabricated glucose biosensor demonstrated its potential in clinical applications, so as to enable the determination of glucose in real serum samples. (C) 2011 Elsevier Ltd. All rights reserved.