Toxicological evaluation for bioremediation processes of TNT-contaminated soil by Salmonella mutagenicity assay

Joon-Seok Park; Byung-Hoon In; Wan Namkoong

Korean Journal of Chemical Engineering, Vol.29, No.8, 1074-1080, August, 2012

DOI10.1007/s11814-011-0286-z Export Citation

Toxicological evaluation for bioremediation processes of TNT-contaminated soil by Salmonella mutagenicity assay

Joon-Seok Park, Byung-Hoon In¹, and Wan Namkoong^{2, †}

Department of Environmental Engineering, Kangwon National University, Gangwon-do 245-711, Korea
¹Department of Soil and Groundwater, Korea Environment Corporation, Environmental Research Complex, Incheon 404-708, Korea
²Department of Environmental Engineering, Konkuk University, Seoul 143-701, Korea

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This research was performed to evaluate the toxicity for composting and slurry phase bioreactor processes of TNT (2,4,6-trinitrotoluene)-contaminated soils by Salmonella mutagenicity assay. For composting, the percentage reductions of final composts in strain TA98 and TA100 with S9 metabolic activation were 90.3-93.7% and 96.7-97.5%, respectively. For slurry phase bioreactor processes, the percentage reductions of final residuals in strain TA98 and TA100 with S9 metabolic activation were 95.0% and 99.1% for anaerobic, 96.2% and 99.2% for anaerobic/aerobic, and 96.6 and 97.4% for anaerobic treatment. Slurry phase treatment showed higher mutagenicity reduction than composting. It was implied that slurry phase treatment was a more effective process than composting in reducing toxicity. This research has the advantage of speed and ease of performance in comparison to testing of other higher life forms due to the shorter life cycles of microorganisms.

Keywords:TNT;Toxicity;Composting;Slurry Phase Bioreactor;Mutagenicity

[References]

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Costa V, Boopathy R, Manning J, Bioresour. Technol., 56(2-3), 273 (1996)
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Gong P, Gasparrini P, Rho D, Hawari J, Thiboutot S, Ampleman G, Sunahara GI, Ecotoxicol. Environ. Safety., 47, 96 (2000)
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Berthe-Corti L. Jacobi H, Kleihauer S, Witte I, Chemosphere., 37, 209 (1998)
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Ames BN, MaCann J, Yamasaki E, Mut. Res., 31, 347 (1975)
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Padda RS, Wang CY, Hughes JB, Bennett GN, Environ. Toxicol. Chem., 19, 2871 (2000)
Honeycutt ME, Jarvis AS, McFarland VA, Ecotoxicol. Environ. Safety., 35, 282 (1996)
Griest WH, Stewart AJ, Tyndall RL, Caton JE, Ho CH, Ironside KS, Caldwell WM, Tan E, Ecotoxicol. Environ. Safety., 15, 1105 (1993)
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[Referenced By]

[1] Schaefer CE, Fuller ME, Condee CW, Lowey JM, Hatzinger PB, J. Contami. Hydrol., 89, 231 (2007)

[2] Esteve-Nunez A, Ramos JL, Environ. Sci. Technol., 32, 3802 (1998)

[3] Urbanski T, Chemistry and technology of explosives, Pergamon Press, Oxford, 4 (1983)

[4] Sunahara GI, Dodard S, Sarrazin M, Paquet L, Hawari J, Greer CW, Ampleman G, Thiboutot S, Renoux AY, Ecotoxicol. Environ. Safety., 43, 138 (1999)

[5] Costa V, Boopathy R, Manning J, Bioresour. Technol., 56(2-3), 273 (1996)

[6] Flokstra BR, Aken BV, Schnoor JL, Chemosphere., 71, 1970 (2008)

[7] Rodgers JD, Bunce NJ, Water Res., 35, 2101 (2001)

[8] Gong P, Gasparrini P, Rho D, Hawari J, Thiboutot S, Ampleman G, Sunahara GI, Ecotoxicol. Environ. Safety., 47, 96 (2000)

[9] Sunahara GI, Dodard S, Sarrazin M, Paquer L, Ampleman G, Thiboutot S, Hawari J, Renoux AY, Ecotoxicol. Environ. Safety., 39, 185 (1998)

[10] Adrian NR, Arnett CM, Chemosphere., 66, 1849 (2007)

[11] Zhang B, Kendall RJ, Anderson TA, Chemosphere., 86 (2006)

[12] Griest WH, Stewart AJ, Tyndall RL, Caton JE, Ho CH, Ironside KS, Caldwell WM, Tan E, Ecotoxicol. Environ. Safety., 15, 1105 (1993)

[13] Griest WH, Tyndall RL, Stewart AJ, Caton JE, Vass A, Ecotoxicol. Environ. Safety., 14, 51 (1995)

[14] Gunderson CA, Kostuk JM, Gibbs MH, Napolitano GE, Ecotoxicol. Environ. Safety., 16, 2529 (1997)

[15] Berthe-Corti L. Jacobi H, Kleihauer S, Witte I, Chemosphere., 37, 209 (1998)

[16] Alexander M, Biodegradation and bioremediation, 2nd Ed., Academic Press (1999)

[17] In BH, Park JS, Namkoong W, Kor. J. Env. Hlth., 32, 578 (2006)

[18] Haug RT, The practical handbook of composting engineering, Lewis Publishers, Ann Arbor, MI (1993)

[19] In BH, Namkoong W, Hwang EY, Kim JD, Park JS, J.Ecotechnol. Res., 13, 303 (2008)

[20] USEPA, Method 8330A: Nitroaromatics and nitroamines by high performance liquid chromatography (HPLC), Test Methods for Evaluating Solid Waste, Physical/Chemical Methods, SW846 Revision 1, Office of Solid Waste, Washington, DC (1998)

[21] Maron DM, Ames BN, Mut. Res., 113, 173 (1983)

[22] Radtke CW, Lehman RM, Roberto FF, Bioreme. J., 4, 57 (2000)

[23] Craig HD, Sisk WE, Nelson MD, Dana WH, Proceedings of the 10th annual conference on hazardous waste research, Kansas State University (1995)

[24] Robertson BK, Jemba PK, Chemosphere., 58, 263 (2005)

[25] Ames BN, MaCann J, Yamasaki E, Mut. Res., 31, 347 (1975)

[26] Won WD, Disalvo LH, James NG, Appl. Environ. Microbiol., 31, 576 (1976)

[27] Tan EL, Ho CH, Griest WH, Tyndall RL, J. Toxicol. Environ.Health., 36, 165 (1992)

[28] Padda RS, Wang CY, Hughes JB, Bennett GN, Environ. Toxicol. Chem., 19, 2871 (2000)

[29] Honeycutt ME, Jarvis AS, McFarland VA, Ecotoxicol. Environ. Safety., 35, 282 (1996)

[30] Griest WH, Stewart AJ, Tyndall RL, Caton JE, Ho CH, Ironside KS, Caldwell WM, Tan E, Ecotoxicol. Environ. Safety., 15, 1105 (1993)

[31] Rocheleau S, Cimpoia R, Paquet L, Koppen IV, Guiot SR, Hawari J, Ampleman G, Thiboutot S, Sunahara GI, Bioreme.J., 3, 233 (1999)