화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.23, No.2, 183-189, April, 2012
Export Citation
땅콩나물 뿌리 추출물의 세포 보호 효과
Cellular Protective Effects of Peanut Sprout Root Extracts
조나래, 박찬일1, 박채원1, 신동한1, 황윤찬1, 김용현1, 박수남†
  • 서울과학기술대학교 정밀화학과, 화장품종합기술연구소
  • 1서울과학고등학교
Na Rae Jo, Chan Il Park1, Chae won Park1, Dong Han Shin1, Yoon Chan Hwang1, Yong Hyun Kim1, and Soo Nam Park†
  • Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology, Seoul 139-743, Korea
  • 1Seoul Science High School, Seoul 110-530, Korea
E-mail:
초록
본 연구에서는 땅콩나물 뿌리 추출물의 세포보호 작용 및 항산화능에 관한 연구를 수행하였다. Rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 땅콩나물 뿌리 추출물의 세포 보호 효과를 측정하였다. 에틸아세테이트 분획(5∼50 μg/mL)은 농도-의존적으로 세포보호 효과를 나타냈으며, 특히 아글리콘(aglycone) 분획은 5∼50 μg/mL의 농도 범위에서 현저한 세포보호 활성을 나타내었다. 그리고 땅콩나물 뿌리의 모든 분획은 지질 과산화 연쇄반응의 차단제인 (+)-α-tocopherol보다도 효과적이었다. Luminol 화학발광법을 이용한 Fe3+-EDTA/H2O2 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 땅콩나물 뿌리 추출물의 활성산소 소거활성을 측정하였다. 추출물 중 에틸아세테이트 분획(OSC50; 1.59 μg/mL)은 강력한 항산화제로 알려진 L-ascorbic acid (1.50 μg/mL)와 비교할 때 유사한 활성산소 소거활성을 보여주었다. 반면에, free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성(FSC50)의 크기는 (+)-α-tocopherol >80% MeOH 추출물 > 아글리콘 분획 > 에틸아세테이트 분획 순으로 나타났다. 이상의 결과들은 땅콩나물 뿌리 추출물이 1O2 및 다른 활성산소종을 소거하고 활성산소종에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로써 작용할 수 있음을 시사한다.
In this study, the cellular protective effect and antioxidative property of peanut sprout root extracts were investigated. Cellular protective effects of peanut sprout root extracts on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction of extracts exhibited a cellular protective effect in a concentration dependent manner. Particularly, the aglycone fraction of extracts showed prominent cellular protective effects in a concentration range (5∼50 μg/mL). They are more effective than that of (+)-α-tocopherol, known as a lipid peroxidation chain blocker. Reactive oxygen species (ROS) scavenging activities (OSC50) of peanut sprout root extracts on ROS generated in Fe3+-EDTA/H2O2 system were investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction of extracts (OSC50; 1.59 μg/mL) showed a similar ROS scavenging activity compare with that of L-ascorbic acid (1.50μg/mL), known as a strong antioxidant. On the other hand, the order of free radical (1,1-diphenyl-2-picrylhydraxyl, DPPH) scavenging activity (FSC50) was (+)-α-tocopherol > 80% MeOH extract > aglycone fraction > ethyl acetate fraction. These results indicate that peanut sprout root extracts can function as an antioxidant in biological systems, particularly skin exposed to solar UV radiation by scavenging 1O2 and other ROS, and to protect cellular membranes against ROS.
Keywords:peanut sprout root extracts;cellular protective effect;photohemolysis;antioxidative activity
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