피부 흡수 증진을 위한 마디풀 추출물 함유 나노에멀젼 제조에 관한 연구

임명선; 박민아; 박수남; Myoung Sun Lim; Min A Park; Soo Nam Park

Applied Chemistry for Engineering, Vol.23, No.2, 222-227, April, 2012

Export Citation

피부 흡수 증진을 위한 마디풀 추출물 함유 나노에멀젼 제조에 관한 연구

Preparation of Nano-emulsion Containing Polygonum aviculare Extract for Enhanced Transdermal Delivery

임명선, 박민아, 박수남 ^†

서울과학기술대학교 정밀화학과, 화장품종합기술연구소

Myoung Sun Lim, Min A Park, and Soo Nam Park^†

Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology, Seoul 139-743, Korea

E-mail:

초록

본 연구에서는 마디풀 추출물의 에틸아세테이트 분획을 함유한 안정한 나노에멀젼을 제조하였고, 이 나노에멀젼의 피부 흡수 증진 효과를 평가하였다. 나노에멀젼은 균질기(homogenizer) 처리 후 고압유화기(microfluidizer)를 이용한 고에너지법으로 제조하였다. 마디풀 추출물의 에틸아세테이트 분획을 함유한 나노에멀젼의 입자 크기와 포집효율을 측정하였다. 평균 입자 크기는 238 nm이었으며 포집 효율은 98% 이상으로 나타났다. 나노에멀젼은 단분산의 입도 분포를 나타냈으며 고압 유화 과정을 거치지 않은 일반 에멀젼보다도 더 안정함을 보여주었다. Franz diffusion cell을 이용하여 마디풀 추출물의 에틸아세테이트 분획을 함유한 나노에멀젼의 피부 투과 실험을 수행하였다. 에틸아세테이트 분획 함유 나노에멀젼은 대조군인 1,3-butylene glycol 용액에서 보다 피부 흡수 및 투과량이 더 큰 것으로 나타났다. 이상의 연구 결과들은 항산화 및 항노화 활성이 큰 마디풀 추출물의 에틸아세테이트 분획을 함유한 나노에멀젼이 안정성 및 피부 투과력이 우수함을 시사한다.

In this study, nano-emulsion containing ethyl acetate fraction of Polygonum aviculare (P. aviculare) extract was prepared and skin permeability of the nano-emulsion was evaluated. Nano-emulsion was prepared using homogenizer and microfluidizer by the high-energy method. The droplet size and loading efficiency of nano-emulsion containing ethyl acetate fraction of P. aviculare extract were determined. The mean droplet size was 238 nm and the loading efficiency was more than 98%. The size distribution of nano-emulsion was a monodispersed form and nano-emulsion was more stable than that of using typical emulsion without using the microfluidizer. The in vitro skin permeation study of nano-emulsion containing ethyl acetate fraction of P. aviculare extract was carried out using Franz diffusion cells. Compared to 1,3-butylene glycol, nano-emulsion showed greater values of cumulative permeation of ethyl acetate fraction from P. aviculare extract. These results indicate that the stability and skin permeability of nano-emulsion containing ethyl acetate fraction of P. aviculare exerting anti-oxidative and anti-aging activities were enhanced.

Keywords:Polygonum aviculare;flavonoids;nano-emulsion;transdermal delivery;Franz diffusion cell

[References]

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Houillot L, Nicolas J, Save M, Charleux B, Li YT, Armes SP, Langmuir, 21(15), 6726 (2005)
Musyanovych A, Mailander V, Landfester K, Biomacromolecules., 87, 212 (1938)
Seifriz W, Science., 87, 212 (1938)
Cajkovac M, Oremovic L, Cajkovac V, Pharmazie., 47, 39 (1992)
Sznitowska M, Zurowska-Pryczkowska K, Janicki S, Jarvinen T, Int. J. Pharm., 184, 115 (1999)
Yue PF, Yuan HL, Zhu WF, Cong LB, Xie H, Liu ZG, Wang LJ, Xiao XH, Biol. Pharm. Bull., 31, 45 (2008)
Barnadas-Rodriguez R, Sabes M, Int. J. Pharm., 213, 175 (2001)
Towata A, Sivakumar M, Yasui K, Tuziuti T, Kozuka T, Lida Y, Ultrason Sonochem., 14, 705 (2007)
Izquierdo P, Feng J, Esquena J, Tadros TF, Dederen JC, Garcia MJ, Azemar N, Solans C, J. Colloid Interface Sci., 285(1), 388 (2005)
Tagne JB, Kakumanu S, Nicolosi RJ, Mol. Pharm., 5, 1055 (2008)
Drug Dev. Ind. Pharm., Calderilla-Fajardo SB, Cazares-Delgadillo J, Villalobos-Garcia R, Quintanar-Guerrero D, Ganem- Quintanar A, Robles R, 32, 107 (2006)
Solans C, Esquena J, Forgiarini A, Morales D, Uso’n N, Izquierdo P, Nano emulsions: formation and properties: surfactants in solution: fundamentals and applications, Surfactant Science Series, eds. Shah D, Moudgil B, Mittal KL, Marcel Dekker, Series, eds. D. Shah, B. Moudgil, K. L. Mittal, Marcel Dekker,, 525 (2002)
Sudol ED, El-Aasser MS, In emulsion polymerization and emulsion polymers, Lovell PA, El-Aasser MS, Eds., John Wiley & Sons, Ltd., Chichester, United Kingdom, 700 (1997)
Marszall L, In nonionic surfactants: surfactant science series, ed. Shick MJ, Marcel Dekker, New York, 23, 493 (1987)
Shinoda K, Saito HJ, Colloid Interface Sci., 26, 70 (1968)
Xu FQ, Liu HB, Luo JG, Zhang JL, Guan HS, Periodical of Ocean University of China, 3 (2010)
Xu FQ, Guan HS, Li GQ, Liu HB, Chromatograpia., 69, 1251 (2009)
Hsu CY, Biol Res., 39, 281 (2006)
Kim HS, Kor. J. Mycol., 8, 1 (1980)
Park SN, Kim SY, Kim MJ, Lim GN, Lim MS, Lee HJ, Park MA, Kim JE, Cellular Protective Effect of Polygonum aviculare Extract and Preparation of Liposome Containing It, IFSCC conference, Bangkok (2011)
Cho WG, J. Korean Oil Chemists’ Soc., 24, 410 (2007)
Baik SS, J. Soc. Comet. Scientist Korea., 19, 127 (1993)
Kyoung KY, Jee UK, Cho WG, J. Kor. Pharm. Sci., 37, 85 (2007)
Kang HS, Kwon SS, Kim BH, Lee BR, Kang KH, Hong JE, Han SH, Chang IS, J. Ind. Eng. Chem., 8(4), 348 (2002)
Cho WG, J. Soc. Comet. Scientist, Korea., 37, 1 (2011)

[Referenced By]

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[1] Won BR, Kang MK, Park SN, J. Soc. Comet. Scientist,Korea., 35, 79 (2009)

[2] Houillot L, Nicolas J, Save M, Charleux B, Li YT, Armes SP, Langmuir, 21(15), 6726 (2005)

[3] Musyanovych A, Mailander V, Landfester K, Biomacromolecules., 87, 212 (1938)

[4] Seifriz W, Science., 87, 212 (1938)

[5] Cajkovac M, Oremovic L, Cajkovac V, Pharmazie., 47, 39 (1992)

[6] Sznitowska M, Zurowska-Pryczkowska K, Janicki S, Jarvinen T, Int. J. Pharm., 184, 115 (1999)

[7] Yue PF, Yuan HL, Zhu WF, Cong LB, Xie H, Liu ZG, Wang LJ, Xiao XH, Biol. Pharm. Bull., 31, 45 (2008)

[8] Barnadas-Rodriguez R, Sabes M, Int. J. Pharm., 213, 175 (2001)

[9] Towata A, Sivakumar M, Yasui K, Tuziuti T, Kozuka T, Lida Y, Ultrason Sonochem., 14, 705 (2007)

[10] Izquierdo P, Feng J, Esquena J, Tadros TF, Dederen JC, Garcia MJ, Azemar N, Solans C, J. Colloid Interface Sci., 285(1), 388 (2005)

[11] Tagne JB, Kakumanu S, Nicolosi RJ, Mol. Pharm., 5, 1055 (2008)

[12] Drug Dev. Ind. Pharm., Calderilla-Fajardo SB, Cazares-Delgadillo J, Villalobos-Garcia R, Quintanar-Guerrero D, Ganem- Quintanar A, Robles R, 32, 107 (2006)

[13] Solans C, Esquena J, Forgiarini A, Morales D, Uso’n N, Izquierdo P, Nano emulsions: formation and properties: surfactants in solution: fundamentals and applications, Surfactant Science Series, eds. Shah D, Moudgil B, Mittal KL, Marcel Dekker, Series, eds. D. Shah, B. Moudgil, K. L. Mittal, Marcel Dekker,, 525 (2002)

[14] Sudol ED, El-Aasser MS, In emulsion polymerization and emulsion polymers, Lovell PA, El-Aasser MS, Eds., John Wiley & Sons, Ltd., Chichester, United Kingdom, 700 (1997)

[15] Marszall L, In nonionic surfactants: surfactant science series, ed. Shick MJ, Marcel Dekker, New York, 23, 493 (1987)

[16] Shinoda K, Saito HJ, Colloid Interface Sci., 26, 70 (1968)

[17] Xu FQ, Liu HB, Luo JG, Zhang JL, Guan HS, Periodical of Ocean University of China, 3 (2010)

[18] Xu FQ, Guan HS, Li GQ, Liu HB, Chromatograpia., 69, 1251 (2009)

[19] Hsu CY, Biol Res., 39, 281 (2006)

[20] Kim HS, Kor. J. Mycol., 8, 1 (1980)

[21] Park SN, Kim SY, Kim MJ, Lim GN, Lim MS, Lee HJ, Park MA, Kim JE, Cellular Protective Effect of Polygonum aviculare Extract and Preparation of Liposome Containing It, IFSCC conference, Bangkok (2011)

[22] Cho WG, J. Korean Oil Chemists’ Soc., 24, 410 (2007)

[23] Baik SS, J. Soc. Comet. Scientist Korea., 19, 127 (1993)

[24] Kyoung KY, Jee UK, Cho WG, J. Kor. Pharm. Sci., 37, 85 (2007)

[25] Kang HS, Kwon SS, Kim BH, Lee BR, Kang KH, Hong JE, Han SH, Chang IS, J. Ind. Eng. Chem., 8(4), 348 (2002)

[26] Cho WG, J. Soc. Comet. Scientist, Korea., 37, 1 (2011)