Selective catalytic reduction (SCR) of NO (nitric oxide) by NH3 (ammonia) at temperatures of 393-523 K over AC (activated carbon) supported V2O5 (vanadium pentoxide) was investigated in this work. The V2O5/AC catalyst was prepared by the pore volume impregnation method. The intrinsic kinetics for this catalyst was first measured in the absence of internal and external diffusions in a fixed-bed microreactor. Then, five intrinsic kinetic models, i.e. the Eley-Rideal model, the Langmuir-Hinshelwood model, the Mars-van Krevelen model, the first-order model, and the power-rate law model, were applied to correlate the experimental data. Among them, the Eley-Rideal model is the most accurate, and the Langmuir-Hinshelwood model is unfeasible for describing SCR of NO by NH3 over the V2O5/AC catalyst. Therefore, the SCR of NO with NH3 over V2O5/AC is likely to follow the Eley-Rideal mechanism in light of intrinsic kinetic measurements. Finally, the kinetic equations were incorporated into a 3D mathematical model for monolithic honeycomb reactor, and it was found that the Eley-Rideal model is more suitable and can provide useful information for future application in the actual industrial plants.