In this correspondence, we report on the first preparation of [Ru(bpy)(2)(dppz)](2+)-intercalated (bpy = 2, 2'-bipyridine; dppz = dipyrido[3,2-a:2',3'-c]phenazine) DNA films on an indium-tin oxide surface via a solution-based self-standing strategy, carried out by the direct mixing of aqueous solutions of both anionic DNA and cationic metallointercalator at a molar ratio of 5:6. The luminescence of a [Ru(bpy)(2)-(dppz)](2+)-intercalated DNA cast film is studied and found to show excellent tunable characteristics by Cu2+ ions and ethylenediaminetetraacetic acid addition.