Evaluation of GENESIS-BCPTM Scaffold Composed of Hydroxyapatite and β-Tricalcium Phosphate on Bone Formation

Dae Hyeok Yang; Ha Na Park; Min Soo Bae; Jung Bok Lee; Dong Nyoung Heo; Won Jun Lee; Young Min Park; Young Hwan Cho; Duck-Su Kim; Il Keun Kwon

Macromolecular Research, Vol.20, No.6, 627-633, June, 2012

DOI10.1007/s13233-012-0090-6 Export Citation

Evaluation of GENESIS-BCPTM Scaffold Composed of Hydroxyapatite and β-Tricalcium Phosphate on Bone Formation

Dae Hyeok Yang, Ha Na Park, Min Soo Bae, Jung Bok Lee, Dong Nyoung Heo, Won Jun Lee¹, Young Min Park², Young Hwan Cho², Duck-Su Kim³, and Il Keun Kwon^†

Department Maxillofacial Biology and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea
¹Department Chemical & Biomolecular Engineering Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, 121-742, Korea
²DIO Co., Busan, 612-020, Korea
³Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea

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The purpose of this study was to evaluate the effects of a GENESIS-BCPTM scaffold composed of hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) on bone formation. The rela tive ratio of components forming GENESIS-BCPTM was investigated by confirming the molar ratio of Ca/P through energy dispersive spectrometer (EDS) analysis. The results being that the scaffold is comprised of 60% HAp and 40% β-TCP. Moreover, scanning electron microscopy (SEM)images showed a unique surface morphology similar to a soccer ball. X-ray diffraction (XRD) analysis showed that the porosity, crystallinity, and Ca/P ratio were 65.5%, 84.9%, and 1.61%, respectively. In vitro tests including cytotoxicity and cell proliferation showed a good biocompatibility of GENESIS-BCPTM. In vivo tests, including mouse skull test, and hematoxylin and eosin stains of GENESIS-BCPTM implanted in rabbit and human subjects showed that the bone graft is effective to accelerate new bone formation. It was concluded that GENESIS-BCPTM can be used as an effective alloplast bone graft.

Keywords:hydroxyapatite (HAp);beta-tricalcium phosphate (β-TCP);GENESIS-BCPTM;mouse skull test;hematoxylin and eosin stain.

[References]

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[Referenced By]

[1] Giannoudis PV, Dinopoulos H, Tsiridis E, Injury Int. J.Care Injured., 36S, S20 (2005)

[2] Cypher TJ, Grossman JP, J. Foot Ankle Surg., 35, 413 (1996)

[3] Carter G, AORN J., 70, 660 (1999)

[4] Araujo M, Linder E, Lindhe J, Clin. Oral Implants Res., 20, 1 (2009)

[5] Gatta AL, Rosa AD, Laurienzo P, Malinconico M, Rosa MD, Schiraldi C, Macromol. Biosci., 5, 1108 (2005)

[6] Centrella M, Horowitz MC, Wozney JM, McCarthy TL, Endocr. Rev., 15, 27 (1994)

[7] Reddi AH, Nat. Biotechnol., 16, 247 (1998)

[8] Takuwa Y, Ohse C, Wang EA, Wozney JM, Yamashita K, Biochem. Biophys. Res. Commun., 174, 96 (1991)

[9] Fodor WL, Reprod. Biol. Endocrinol., 1, 102 (2003)

[10] Miller CL, Imren S, Antonchuk J, Kalberer C, Fabry ME, Mol. Ther., 6, 422 (2002)

[11] Knabe C, Stiller M, Berger G, Reif D, Gildenhaar R, Howlett CR, Zreiqat H, Clin. Oral Implants Res., 16, 119 (2005)

[12] Yamada S, Heymann D, Bouler JM, Daculsi G, Biomaterials., 18, 1037 (1997)

[13] Legeros RZ, Lin S, Rohanizadeh R, Mijares D, Legeros JP, J. Mater. Sci. Mater. Med., 14, 201 (2003)

[14] Ducheyne P, Qiu Q, Biomaterials., 20, 2287 (1999)

[15] Yoshikawa H, Myoui A, J. Artif. Organs., 8, 131 (2005)

[16] Murugan R, Rao KP, Macromol. Res., 11(1), 14 (2003)

[17] Ma PX, Zhang R, Xiao G, Franceschi R, J. Biomed.Mater. Res., 54, 284 (2001)

[18] Markocic E, Skerget M, Knez Z, J. Supercrit. Fluids, 55(3), 1046 (2011)

[19] Bae MS, Yang DH, Lee JB, Heo DN, Kwon YD, Lee WJ, Yoon IC, Choi K, Hong JH, Kim GT, Choi YS, Hwang EH, Kwon IK, Biomaterials., 32, 8161 (2011)

[20] Yang DH, Bae MS, Lee JB, Heo DN, Kim JE, Lee WJ, Park YM, Cho YH, Kwon IK, Biomater. Res., 15, 85 (2011)

[21] Kokubo T, Takadama H, Biomaterials., 27, 2907 (2006)

[22] Freeman TA, Patel P, Parvizi J, Antoci V, Shapiro IM, J. Orthop. Res., 27, 673 (2009)

[23] Ullmark G, Obrant KJ, J. Arthroplasty., 17, 150 (2002)