화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.23, No.3, 279-283, June, 2012
Export Citation
산 및 효소 가수분해를 이용한 홍조류로부터 바이오 에탄올 생산
Production of Bio-ethanol from Red Algae by Acid Hydrolysis and Enzyme Treatment
최수정, 이성목, 이재화†
  • 신라대학교 의생명과학대 생명공학과
Soo-Jeong Choi, Sung-Mok Lee, and Jae-Hwa Lee†
  • Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University, Busan 617-736, Korea
E-mail:
초록
화석연료로 인한 환경오염 등의 문제를 해결하기 위해서 다양한 원료를 이용하여 바이오 에탄올 생산에 대한 연구가 진행되고 있다. 해조류 중에 홍조류는 agar, carrageenan, porphyran으로 구성되어 있어 산 처리를 통해 바이오에탄올 생산에 유용한 바이오매스로 전환이 가능하다. 본 연구는 홍조류의 가수분해물을 이용하여 바이오에탄올 생산의 최적조건을 찾으려고 한다. 바이오에탄올 생산하기 위해 전처리 된 우뭇가사리에 Saccharomyces cerevisiae KCCM112를 접종해 발효하였다. 우뭇가사리 가수분해의 최적조건은 1.5% H2SO4를 121 ℃에서 30 min 반응시켰을 때 7.04 g/L의 galactose와 1.94 g/L의 glucose가 생산되었다. 그리고 CH3COOH의 경우 2.0% 농도로 처리하였을 때, galactose 0.75 g/L가 생산되었다. 이와 반대로 도박에서는 H2SO4 1.5%를 처리하였을 때 galactose를 6.38 g/L 생산하였으며, CH3COOH을 처리했을 때 0.368 g/L이 생산되었다. 우뭇가사리에서 에탄올 생산은 1.0% H2SO4를 121 ℃에서 30 min 간 처리하였을 때 가장 높았으며, 96 h 배양하였을 때 3.77 g/L의 에탄올을 생산했다.
Bio-ethanol production research using various material has been problemed for solving problems of environment pollution caused by fossil fuels. Red-algae consists of agar, carrageenan, and porphyran. If it is treated by acid, it is able to change useful bio-mass for bio-ethanol. In this study, we found an optimal condition for bio.ethanol production from acid hydrolysate in red-algae. To produce bio-ethanol, Saccharomyces cerevisiae KCCM1129 inoculated to acid hydrolysate of Gelidium amansii. The optimal condition for Gelidium amansii hydrolysis was found to be 30 min reaction at H2SO4 concentration of 1.5% and 121 ℃. At this condition, its produced to 7.04 g/L galactose and 1.94 g/L glucose. And acetic acid concentration of 2.0% in agar produced 0.75 g/L galactose. In contrast, Pachymeniopis elliptica was treated with H2SO4 concentration of 1.5%, it produced 6.38 g/L galactose. And Pachymeniopis elliptica treated with acetic acid concentration of 2% produced 0.368 g/L galactose. The optimal condition of ethanol production was found to be 96 h reaction at H2SO4 concentration of 1.0% and 30 ℃, which produced 3.77 g/L ethanol.
Keywords:bio-ethanol;pretreatment;Saccharomyces cerevisiae;Gelidium amansii
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