화학공학소재연구정보센터
Inorganic Chemistry, Vol.39, No.2, 281-288, 2000
High-frequency EPR study of the ferrous ion in the reduced rubredoxin model [Fe(SPh)(4)](2-)
High-frequency (94-371 GHz) EPR data are reported for powdered samples of [PP4](2)[Fe(SPh)(4)], an accurate model for the reduced site of rubredoxins. This is the first HFEPR investigation of an S = 2 ferrous complex, illustrating the utility of this technique for the investigation of integer-spin systems. A full-matrix diagonalization approach is used to simulate spectra over the 94-371 GHz frequency range, providing the spin-Hamiltonian parameters g, D, and E. It is observed that g is anisotropic, characterized by g(x) = g(y) = 2.08 and g(z) = 2.00, and that D = +5.84 cm(-1) and E = +1.42 cm(-1), where the uncertainty in each parameter is estimated as +/-2%. The spin-Hamiltonian for [PPh4](2)[Fe(SPh)(4)]' is related to fundamental properties, such as the crystal-field splitting and the spin-orbit coupling of Fe2+. It is shown that the conventional spin-Hamiltonian accurately represents the electronic structure of the Fe2+ ion in this molecule. Through a comparison with Fe(SPh)(4)(PPh4)(2), the zero-field splitting of the Fe2+ site in reduced rubredoxin is estimated to be D = +5.3 cm(-1) and E = +1.5 cm(-1). This is one of the few HFEPR investigations of a rhombic, high-spin system; as such, it is a step toward the eventual investigation of similar Fe2+ sites in proteins.