This study focuses on the synthesis and characterization of the inclusion complex of beta-Cyclodextrin (beta-CD) with dicationic ionic liquid, 3,3'-(1,4-Phenylenebis [methylene]) bis(1-methyl-1H-imidazol-3-ium) di(bromide) (PhenmimBr). The inclusion complex was prepared at room temperature utilizing conventional kneading technique. Proton ((1)H) NMR and 2D ((1)H-(1)H) COSY NMR were the primary characterization tools employed to verify the formation of the inclusion complex. COSY spectra showed strong correlations between protons of imidazolium and protons of beta-CD which indicates that the imidazolium ring of PhenmimBr has entered the cavity of beta-CD. UV absorption indicated that beta-CD reacts with PhenmimBr to form a 2:1 beta-CD-PhenmimBr complex with an apparent formation constant of 2.61 x 10(5) mol(-2) L(2). Other characterization studies such as UV, FT-IR, XRD, TGA, DSC and SEM studies were also used to further support the formation of the beta-CD-PhenmimBr inclusion complex.