Electrochemical deposition method was employed for the fabrication of rhodium-palladium (Rh-Pd) particles on the glassy carbon electrode (GCE) and indium tin oxide (ITO) electrode surface. Surface morphological analysis of Rh-Pd film has been carried out using scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis. Here, the electrodeposited Rh-Pd particles were found in the average size range of 30-200 nm. The electrochemical activities of the Rh-Pd film have been investigated using cyclic voltammetry (CV) and electrochemical impedance spectroscopic (EIS) analysis. The Rh-Pd particles-modified GCE successfully detects the hydrogen peroxide (H(2)O(2)) (in pH 7.0 phosphate buffer solution (PBS)) in the linear range in the lab (10-460 mu M) and real samples (10-340 mu M). The Rh-Pd particles-modified GCE possesses the good sensitivity and selectivity for the detection of H(2)O(2) in lab and real samples.