Aims: In 2005, a Francisella sp. was isolated from diseased cultured giant abalone (Haliotis gigantea) in Japan. The aim of this study was to clarify the taxonomic status of this Francisella sp. Shimane-1 isolate in relation to the four described Francisella species. Methods and Results: The 16S rRNA gene and several housekeeping genes of the Shimane-1 were compared to isolates of the four recognized species within the Francisella genus. DNA-DNA hybridization (DDH) and biochemical profile comparison were performed with the two phylogenetically closely related species, Francisella philomiragia and Francisella noatunensis. Results show that the Shimane-1 is genetically different from all described Francisella species and differs phenotypically from F. philomiragia and F. noatunensis. The average DDH similarity of Francisella sp. Shimane-1 to F. noatunensis ssp. noatunensis (NCIMB14265(T)) and to F. philomiragia (DSM7535(T)) was 49.2 and 61%, respectably, clearly supporting the establishment of Shimane-1 as a new species within the Francisella genus. Conclusions: The phenotypic and genetic results presented in this study suggest the establishment of Shimane-1 as a novel species, for which the name Francisella halioticida sp. nov. (=LMG26062(T), =DSM23729(T)) is proposed. Significance and Impact of the Study: This study clarifies the taxonomic position and characteristics of a novel mollusc pathogenic Francisella species.