Journal of Bioscience and Bioengineering, Vol.111, No.2, 117-120, 2011
Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter
Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L edodes transformation. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.