This study investigates the effects of gemini and conventional cationic surfactants on the cloud point (CP) of the non-ionic surfactant Triton X-100 (TX-100) in aqueous solutions. Instead of visual observation, a spectrophotometer was used for measurement of the cloud point temperatures. The thermodynamic parameters of these mixtures were calculated at different cationic surfactant concentrations. The gemini surfactants of the alkanediyl-alpha-omega-bis (alkyldimethylammonium) dibromide type, on the one hand, with different alkyl groups containing m carbon atoms and an ethanediyl spacer, referred to as "m-2-m" (m = 10, 12, and 16) and, on the other hand, with -C(16) alkyl groups and different spacers containing s carbon atoms, referred to as "16-s-16" (s = 6 and 10) were synthesized, purified and characterized. Additions of the cationic surfactants to the TX-100 solution increased the cloud point temperature of the TX-100 solution. It was accepted that the solubility of non-ionic surfactant containing polyoxyethylene (POE) hydrophilic chain was a maximum at the cloud point so that the thermodynamic parameters were calculated at this temperature. The results showed that the standard Gibbs free energy (Delta G(cp)(o)), the enthalpy (Delta H(cp)(o)) and the entropy (Delta S(cp)(o)) of the clouding phenomenon were found positive in all cases. The standard free energy (Delta G(cp)(o)) increased with increasing hydrophobic alkyl chain for both gemini and conventional cationic surfactants; however, it decreased with increasing surfactant concentration. (C) 2011 Elsevier Ltd. All rights reserved.