Microstructural evolution during creep of a hot extruded Al-Cu-Mg-Fe-Ni (2D70) Al-alloy was investigated in this study using transmission electron microscopy (TEM). The samples for creep test were carried out two-stage homogenization, followed by extruding. The creep ultimate strength dropped and the temperature increased gradually from 312 to 117 MPa and from 423 to 513 K, respectively. The microstructural observation for the crept samples showed that the S' phase coarsened with increased creep temperature and the aging precipitates transformed from SaEuro(3) phase to S' phase during creep process. Meanwhile, excess solute atoms in supersaturated solid solution dynamically precipitated to further form finer S' phase and SaEuro(3) phase, which pinned the dislocations and impeded the dislocation movements. Large amount of dislocations piled up around the micron-scale Al(9)FeNi phase, and a lot of dislocation walls were generated along < 220 > orientation. S phase accumulates around these defects. The interaction between dislocations and precipitates was beneficial for the improved performances at elevated temperature.