A cobalt(II) tetra-neopentyloxy phthalocyanine-multi-walled carbon nanotubes (CoTNPPc-MWNTs) composite was synthesized and characterized by UV-Vis spectra and transmission electron microscopy. The CoTNPPc-MWNTs glassy carbon electrode (CoTNPPc-MWNTs/GCE) was prepared by drop coating. The electrocatalytic performance of the chemically modified electrode was investigated for oxidation of ascorbic acid (AA). It was found that in phosphate buffer solution at pH = 6.60, the chemically modified electrode exhibited excellent electrocatalytic activity toward the oxidation of AA. The oxidation peak current increased linearly with the concentration of AA in the range of 10 mu M-1.6 mM within the detection limit of 5 mu M and low response time of 4 s.