In this work, we have reported the application of less familiar ethylene glycol-AOT reverse micelle for the synthesis of silver nanoparticle using glucose as mild reducing agent and isooctane as the continuous media. We have also studied the pure ethylene glycol-AOT reverse micelle and the perturbed reverse micelle (containing silver nanoparticle in its womb) through solvation dynamics measurement using steady-state and time-resolved fluorescence spectroscopy. Finally, we compared both of the results to get the valuable information about the perturbed reverse micellar system containing silver nanoparticle. Through the work, we found that in the pure reverse micellar system, with increasing ethylene glycol loading, solvation time was decreasing and anisotropy value became slower. In the perturbed reverse micellar system (containing silver nanoparticle) having the same environmental state, that is, at the same ethylene glycol content, solvent and rotational relaxation became slower and faster, respectively.