Interaction of gelatin (G) with room temperature ionic liquids (ILs), 3-methyl-1-octylimidazolium chloride [C(8)mim][Cl] and 1-butyl-3-methylimidazolium octylsulfate [C(4)mim][C8OSO3], have been investigated through tensiometry, conductivity, steady-state fluorescence, turbidity, and dynamic light scattering (DLS). We have observed that the nature of interactions in G-[C(8)mim][Cl] system are remarkably different as compared to G-[C(4)mim][C8OSO3] system. At low concentrations, much below the critical micelle concentration (cmc) of IL, the IL monomers are adsorbed at the native G at the interface forming G-IL (monomer) complex, whereas both the monomers and lower IL aggregates are interacted with G in bulk leading to G-IL (aggregate) complex. The increased hydrophobic character of the G-IL complexes is evidenced from pyrene fluorescence. Turbidity measurements showed interestingly distinguished coacervation characteristics in the investigated systems. In case of G-[C(4)mim][C8OSO3] system, the coacervates dissolve in the free micellar solution whereas G-[C(8)mim][Cl] coacervates remain stable up to very high concentration. DLS provided useful information about the changes in size of gelatin and the nature of interactions between gelatin and ILs. Thermodynamic parameters of micellization with and without gelatin have been derived and compared.