The purpose of this study was to formulate and evaluate the physicochemical properties and efficacy of an oral melanoma vaccine. Blood, feces and vaginal wash were collected weekly and analysed by ELISA. The mortality and diameter of the tumors were determined using a vernier caliper. The oral melanoma vaccine microparticles demonstrated desirable particle size, product yield, and zeta potentials. In addition, FT-IR and DSC studies revealed that there was no significant degradation in microencapsulated extra-cellular antigen (ECA). The oral vaccine group showed 25% greater survival rate compared to the control in the efficacy and challenge studies.