Some 180 core and cuttings samples of shales and limestones from the Middle Jurassic - Late Cretaceous succession (Khatatba, Masajid, Alam El-Bueib, Alamein, Kharita, Bahariya and Abu Roash Formations) were collected from wells Ja 27-2, Tarek-1 and Jb 26-1 in the central, structurally-low part of the Shushan Basin and from well Lotus-1 in the structurally-elevated western part of the basin. All samples were screened for total organic carbon (TOC) content. Selected samples were then analyzed by Rock-Eval pyrolysis, and extracted for biomarker analyses. Visual kerogen analysis and vitrinite reflectance measurements were also undertaken and oil - source rock correlations were attempted. The results indicate that the thermal maturity of the samples can be correlated closely with burial depth. Samples from the central part of the basin are more mature than those from the west. Samples from the central part of the basin (except those from the Albian Kharita Formation) have reached thermal maturities sufficient to generate and expel crude oils. Extracts from the Middle Jurasic Khatatba and Early Cretaceous Alam El-Bueib Formations can be correlated with a crude oil sample from well Ja 27-2. In well Lotus-1 in the west of the basin, four distinct organic facies can be recognized in the Jurassic-Cretaceous interval. One of the facies ("facies 4") has a sufficiently high TOC content to act as a source rock. Thermal maturities range from immature to peak oil generation, and the top of the oil window occurs at approximately 8000 ft.