Collagen α-Tocopherulate for Topical Applications: Preparation, Characterization, and Antioxidant Activity Evaluation

Sonia Trombino; Roberta Cassano; Teresa Ferrarelli; Benedetta Isacchi; Anna Rita Bilia; Nevio Picci

Macromolecular Research, Vol.20, No.9, 939-943, September, 2012

DOI10.1007/s13233-012-0129-8 Export Citation

Collagen α-Tocopherulate for Topical Applications: Preparation, Characterization, and Antioxidant Activity Evaluation

Sonia Trombino, Roberta Cassano^†, Teresa Ferrarelli, Benedetta Isacchi¹, Anna Rita Bilia¹, and Nevio Picci

Department of Pharmaceutical Sciences, University of Calabria, 87036, Arcavacata di Rende, Cosenza, Italy
¹Department of Pharmaceutical Sciences, University of Florence, I-50019, Sesto Fiorentino, Firenze, Italy

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The aim of this work was to synthesize collagen α-tocopherulate for topical treatments. Collagen is a natural polymer largely applied to enhance wound repair, and α-tocopherol is a known antioxidant used for the treatment of various skin diseases. Linking the two through covalent bonds could improve α-tocopherol transport and metabolic stability, reduce the rate of degradation, and ensure a longer persistence than free antioxidant. The new biopolymer was prepared by a solid-phase synthesis employing a carboxypolystyrene resin. Its antioxidant activity in rat liver microsomal membranes was also evaluated. The obtained collagen-based biomaterial represents a new prodrug of α-tocopherol that could be used for various biomedical applications.

Keywords:solid phase synthesis;α-tocopherol protein conjugate;antioxidant activity.

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[1] Pitarresi G, Palumbo FS, Cavallaro G, Fare S, Giammona G, J. Biomed. Mater. Res., 87A, 770 (2008)

[2] Nair LS, Laurencin CT, Prog. Polym. Sci., 32, 762 (2007)

[3] Timpl R, in Extracellular Matrix Biochemistry, Piez KA, Reddi AH, Eds., Elsevier, New York, 159 (1984)

[4] Lee CH, Singla A, Lee Y, Int. J. Pharm., 221, 1 (2001)

[5] Olsen D, Yang C, Bodo M, Chang R, Adv. Drug Deliv. Rev., 55, 1547 (2003)

[6] Vin F, Teot L, Measume SJ, Wound Care., 11, 335 (2002)

[7] Thornton JF, Rohrich RJ, Plast. Reconstr. Surg., 116, 677 (2005)

[8] White MJ, Heckler FR, Clin. Plast. Surg., 17, 473 (1990)

[9] Sukla A, Rasik AM, Patnaik GK, Free Radic. Res., 26, 93 (1997)

[10] Albericio F, Chinchilla R, Dodsworth DJ, Najera C, Org. Prep. Proced. Int., 33, 203 (2001)

[11] Sabatino G, Mulinacci B, Alcaro MC, Chelli M, Rovero P, Papini AM, Lett. Pept. Sci., 9, 119 (2002)

[12] Tatum VL, Changoit C, Chow CK, Lipids., 25, 226 (1990)

[13] Trombino S, Serini S, Di Nicuolo F, Celleno L, Ando S, Picci N, Calviello G, Palozza P, J. Agric. Food Chem., 52, 2411 (2004)

[14] Bradford MM, Anal. Biochem., 72, 248 (1976)

[15] Hagerman AE, Riedl KMJ, Alexader G, Sovik KN, Ritchard NT, Hartzfeld T, Riechel WL, J. Agric. Food Chem., 46, 1887 (1998)

[16] Cassano R, Trombino S, Ferrarelli T, Muzzalupo R, Tavano L, Picci N, Carbohydr. Polym., 78, 639 (2009)

[17] Cassano R, Trombino S, Muzzalupo R, Tavano L, Picci N, Eur. J. Pharm. Biopharm., 72, 232 (2009)

[18] Le Corre D, Bras J, Dufresne A, Biomacromolecules, 11(5), 1139 (2010)

[19] Duan X, McLaughlin C, Griffith M, Sheardown H, Biomaterials., 28, 78 (2007)

[20] Radhika M, Sehgal PK, J. Biomed. Mater. Res., 35, 497 (1997)

[21] Gruessner M, Clemens PV, Pahlplatz P, Sperling J, Sperling P, Am. J. Surg., 182, 502 (2001)