Lipid giant vesicles (GVs) exhibit biologically relevant morphological dynamics such as growth and division under certain conditions without any sophisticated molecular machineries employed by the current organisms. Nonequilibrium conditions are essential for the emergence of dynamic behaviors, which are normally generated by the addition of stimulating materials or by the change of some physical conditions. Therefore, an experimental method that allows flexible control of external conditions is desirable. Here we report a new and simple perfusion device for light microscopy observation that simultaneously realizes such control and tracking of individual phospholipid GVs for the long-term. We apply this device to the study of the morphological dynamics of POPC-based giant multilamellar vesicles (GMVs) under a monotonic and gradual increase of surfactant concentration; thereby we reveal the existence of multiple pathways in the slow solubilization processes, whose frequencies depend on the compositions of GMVs. This perfusion device would offer an unprecedented control of external conditions in the studies of GVs and might help us characterize the physicochemical origins of rich morphological dynamics of living cells.