The Joule heating effect is inevitable in electrophoresis operations. To assess its influence on the performance of electrophoresis, we consider the case of a charge-regulated particle in a solution containing multiple ionic species at temperatures ranging from 298 to 308 K. Using an aqueous SiO(2) dispersion as an example, we show that an increase in the temperature leads to a decrease in both the dielectric constant and the viscosity of the liquid phase, and an increase in both the diffusivity of ions and the particle surface potential. For a particle having a constant surface potential, its electrophoretic mobility is most influenced by the variation in the liquid viscosity as the temperature varies, but for a charged-regulated particle both the liquid viscosity and the surface potential can play an important role. Depending upon the level of pH, the degree of increase in the mobility can be on the order of 40% for a 5 K increase in the temperature. The presence of double-layer polarization, which is significant when the surface potential is sufficiently high, has the effect of inhibiting that increase in the mobility. This implies that the influence of the temperature on the mobility of the particle is most significant when the pH is close to the point of zero charge.