The nucleotide sequence of the pathogenesis-related protein 1(-) gene was obtained from using RT-PCR. Restriction enzyme cutting sites of RI and I were introduced to the ORF fragments of -, they were then linked together with pET-30a (+) and transformed into BL21 (DE3). The target protein was induced by 1.5 mM IPTG, at 37A degrees C for 4 h. The expressed protein was purified by Ni-NTA and an anti-NbPR-1 polyclonal antibody was prepared using rabbits. The antibody could detect the expression of PR-1 in and other plants. NbPR-1 protein has four alpha-helices and two beta-sheets by homology modeling. Furthermore, the purified NbPR-1 protein exhibited a broad-spectrum antifungal activity.