SQR-21, which is antagonistic against , is used as a biocontrol agent and, when mixed with organic substances for solid fermentation, produces a bioorganic fertilizer. The spores of prepared at different temperatures were characterized with respect to the dipicolinic acid content, heat resistance, fatty acid composition and germination. Spores prepared at 37A degrees C showed higher heat resistance than those prepared at 25 and 30A degrees C. However, the germination rate was negatively correlated with the sporulation temperature. The maximum germination rate of the spores prepared at 25A degrees C was 1.3-times higher than the spores prepared at 30A degrees C. The sporulation temperature thus affects the resistance and germination properties of spores. These results are useful for the production of improved bio-organic fertilizer.