The adsorption of miglitol on the D001 resin in a fixed bed was studied at different flow rates, temperatures, and height-to-diameter ratios (H/D). The exhaustion time decreased and the height of the mass transfer zone (HMTZ) increased with increasing flow rate. The surface area of the adsorbent increased with decreasing solution flow rate. Breakthrough curves at different flows were described by the Thomas, YoonNelson, and Adams-Bohart models. The rate constants for the kinetic models increased with increasing flow rate. The theoretical and experimental times required for 50?% adsorbate breakthrough slightly differed at varying flow rates. The change trend in the values of the maximum volumetric adsorption capacity was the same as that of the values at the maximum adsorption capacity of the D001 resin. The temperature did not obviously affect the breakthrough curves. The slope of the breakthrough curve decreased and the HMTZ increased with increasing H/D ratio. The adsorption capacities of the fixed bed for H/D = 7.6 and 0.7 were greater than that for H/D?=?1.5.