Mixing of a passive scalar inside a pitched blade turbine (PBT) impeller stirred tank (STR) is studied using large-eddy simulation (LES) coupled with the immersed boundary method (IBM) for resolving moving interfaces. Mixing time is calculated based on the 95% homogenization of the scalar over the entire tank volume. Growth rate of the unmixed tracer volume is observed in order to identify the effects of low frequency macroinstability (MI) oscillations. Mixing time is significantly reduced when the STR flow is perturbed using a step-change in the impeller speed with a specific MI frequency. The enhancement in turbulent kinetic energy and changes in mean flow field due to the perturbation is observed. The spatio-temporal behavior of the large-scale mixing structures for the fixed impeller-speed case and the perturbed case are compared. The mechanism of mixing enhancement is further explored by observing dynamic changes in the concentration distribution and the velocity field over a perturbation cycle. Penalty in power requirement due to perturbation is calculated. (C) 2011 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.