High-energy (116 keV) synchrotron radiation X-ray fluorescence analysis has for the first time been applied to the quantitative analysis of trace heavy elements of geological samples. Calibration curves for the determination of trace heavy elements were obtained by measuring 7 reference rock samples and showed good linearity because of the high penetrating power of the high-energy X-rays. It is found that this method is suitable for rapid and nondestructive quantitative characterization of trace heavy elements in trace amount of soil samples for forensic investigation.