The paper described experimental investigation of heat transfer and single-phase pressure drop through tubes with different rotor-assembled strands inserted in the Reynolds number range of 800-9000 with lubricant as working fluid. In the experiment, fixed mounts were employed to eliminate the entrance effect. The experimental results showed that the employment of fixed mounts led to a visible bias of friction factor in the laminar regime while it could not affect the Nusselt numbers significantly. Experiment for the tube inserted with rotors-assembled strand showed remarkable improvement for heat transfer with the Nusselt number increased by 200%-225% in the laminar regime and 125%-160% in the transitional regime. Meanwhile, the friction factor increased inevitably by 200%-300% within the same range of Reynolds number. The comparison of different rotor-assembled strands inserted tubes and plain tube showed that the heat transfer benefited from the increase of the diameter of rotor-assembled strand with the same lead and the decrease of the lead of rotor-assembled strand, so does the friction factor. Based on experimental data and thorough multivariant linear normal regression method, the correlations of average Nusselt number and friction factor are established.