Two psychophysical experiments were carried out to investigate whether or not colour emotion responses would change with the advance of the viewer's age. Two forms of stimuli were used: 30 single colours (for Experiment 1) and 190 colour pairs (for Experiment 2). Four word pairs, warm/cool, heavy/light, active/passive, and like/dislike, were used to assess colour emotion and preference in Experiment 1. In Experiment 2, harmonious/disharmonious was also used in addition to the four scales for Experiment 1. A total of 72 Taiwanese observers participated, including 40 (20 young and 20 older) for Experiment 1 and 32 (16 young and 16 older) for Experiment 2. The experimental results show that for single colours, all colour samples were rated as less active, less liked, and cooler for older observers than for young observers. For colour combinations, light colour pairs were rated as less active and cooler for older observers than for young observers; achromatic colour pairs and those consisting of colours in similar chroma were rated as cooler, less liked and less harmonious for older observers than for young observers. The findings may challenge a number of existing theories, including the adaptation mechanism for retaining consistent perception of colour appearance across the lifespan, the modeling of colour emotion based on relative colour appearance values, and the additive approach to prediction of colour-combination emotion. (c) 2011 Wiley Periodicals, Inc. Col Res Appl, 2012; Published online 1 March 2011 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/col.20672