In the present work, polyester film and fabric were exposed to dielectric barrier discharge in air at atmospheric pressure to increase wettability and dye uptake properties. The surface of dielectric barrier discharge treated samples was analyzed using contact angle goniometer, scanning electron microscope, and Fourier transform infrared spectrometer. The plasma was characterized electrically and optically to understand plasma chemistry. Significant improvement in wettability of polyester substrate and substantial decrease in water contact angle at surface of polyester film after plasma treatment were attributed to the formation of polar functional groups and is due to morphological changes. The aging effect was also studied on plasma treated polyester surface. Polyester fabric was dyed with natural dispersive dye eco-alizarin. K/S value has increased significantly from 5.29 for untreated to 9.25 for air plasma treated polyester fabric, which clearly indicate an increase in color intensity on plasma exposed polyester fabric as well as an increase in dye uptake in comparison to untreated fabric.