The selective oxidative desulfurization was conducted with a feedstock of model gasoline consisting of thiophene and n-octane in an H2O2 center dot HCOOH oxidative system over a Ti-MCM-41 catalyst. The operations were designed by the orthogonal design method with an L-9 (3(4)) table. Experimental results show that the factors influencing the rate of desulfurization are reaction temperature, the reaction time, and the volume percent content of the hydrogen peroxide in order Under the optimized operation conditions over Ti-MCM-41 catalyst: H2O2 volume fraction of 3% (v), reaction time of 40 min and reaction temperature of 50 degrees C, the removal ratio of thiophene was 95.6%, and the gasoline yield was 98.7%. The activity of the catalyst has no dramatic deactivation after reusing it five times. In addition, the kinetics study indicates that this reaction is a pseudo first-order reaction with the activation energy of Ea = 40.18 kJ/mol.