Wrinkles with two distinct wavelengths formed sequentially on the same surface are investigated. A series of aligned wrinkles are formed through local strain application on a partially crosslinked elastomer. After the formation of these primary wrinkles, the elastomer is fully crosslinked, and a mechanical compressive strain is applied to the sample orthogonal to the primary wrinkles. This mechanical strain results in smaller secondary wrinkles superimposed on the larger primary aligned wrinkles. Resulting biaxial morphologies suggest that the primary pattern directs the formation of the smaller wrinkles. The modulus mismatch of the substrate on primary and secondary wrinkle formation dictates the ratio between the two resulting wavelengths, as well as the specific biaxial morphologies, ranging from zigzag ridges to ellipsoidal bumps or corn-on-the-cob structures to the classic herringbone. The sequential strain wrinkling process has the potential to be used on an industrial scale for the facile formation of surface topography with two discrete, tunable lateral dimensions over large surface areas. (c) 2012 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2012