The use of fabrics with antibacterial properties for commodity applications can provide numerous advantages such as a reduction in the release of odors due to bacterial proliferation in sweat and a reduction in the development of skin hypersensitivity reactions due to microorganisms trapped into the fabrics. Silver is one of the most effective antibacterial agents used for the high degree of biocompatibility and for its long-term antibacterial effectiveness against many different bacterial strains. In this study, an innovative technique for the deposition of nanosilver antibacterial coating on woolen fiber was analyzed. In particular, fabrics woven with different percentages of silver-treated fibers were compared to determine the best ratio preserving the antibacterial activity and optimizing the cost-effectiveness of the final product. Scanning electron microscopy revealed a uniform distribution of silver nanoclusters on the fibers. The impressive silver coating stability and durability were demonstrated after several washing cycles through thermogravimetric analysis. The antimicrobial activity of the silver-treated substrates was evaluated by antibacterial tests on Escherichia coli. A very strong antibacterial activity was found even in presence of the lower silver content; therefore, a blend of coated and uncoated fibers is proposed for practical applications. (c) 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012