The aim of this paper is to carry out a comparative study with regard to energy consumption and greenhouse gas emissions, in respect of two types of engines with three different fuels. The fuels analysed are diesel, biodiesel 30% (B30) and compressed natural gas (CNG). The engines tested were a spark ignition engine (Otto cycle) and two compression ignition engines (Diesel cycle), the first fed with CNG and the last two with B30 and diesel. What is new about this study is its scope of application concerning refuse collection services in the city of Madrid. The tests were carried out on refuse trucks of the FCC Company along actual urban routes in the city of Madrid. Also taken into account were the energy input and the greenhouse gases emitted for each of the paths taken by the fuels analysed, from resource recovery to delivery to the vehicle tank. (C) 2008 Elsevier Ltd. All rights reserved.